rat monoclonal IgG2a; Blimp-1 Antibody (6D3) is an IgG2a rat monoclonal Blimp-1 antibody (also designated PRDM1 antibody, PR/SET domain 1 antibody, or BLIMP1 antibody) that detects the Blimp-1 protein of mouse, rat and, to a lesser extent, human origin by WB, IP, IF and IHC(P). Blimp-1 Antibody (6D3) is available as both the non-conjugated anti-Blimp-1 antibody form, as well as multiple conjugated forms of anti-Blimp-1 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. The development and differentiation of plasma cells, which are terminally differentiated B-cells, are induced by Blimp-1 (B lymphocyte-induced maturation protein, also designated PRDI-BF1). Blimp-1 is a transcriptional repressor that localizes to the nucleus and is considered a master regulator of terminal B-cell development. Alone, Blimp-1 is sufficient to trigger terminal B-cell differentiation. Blimp-1 upregulates the expression of syndecan-1 and J chain, represses IFN-b gene transcription and associates with HDAC to recruit it to DNA, thereby repressing c-myc. Blimp-1 is expressed during the late stages of B-cell differentiation in immunoglobulin-secreting plasma cells, as well as in long-lived, bone marrow plasma cells. The expression of Blimp-1 defines a checkpoint beyond which fully activated B cells proceed to the plasma cell stage, whereas immature and partially activated cells are eliminated.