A scalable miniature circular GenCircle™ dsDNA production system for gene and cell therapy

 

Circular plasmid DNA has traditionally been used as a stable and efficient vector for gene expression and HDR-mediated gene insertion. These vectors typically carry at least one antibiotic-resistant gene to ensure that the positive clone is accurately selected and genetically stable. However, for therapeutic applications, introducing antibiotic-resistant genes via exogenous plasmid DNA risks integration into the human genome causing resistance in patients. Therefore, eliminating the presence of antibiotic-resistance genes in the manufacture of plasmid DNA has been of interest.

 

Additionally, smaller-sized plasmid DNA significantly improves transfection and expression efficiency. However, a reduced backbone poses challenges when manufacturing circular dsDNA.

 

In this presentation, we will introduce GenCircle, our newly developed proprietary miniature circular dsDNA. GenCircle dsDNA is antibiotic resistance gene free with only 429bp backbone size and an easily scalable manufacturing process. We will also discuss how to best design your GenCircle dsDNA construct, as well as data on its improved performance in T cell engineering and in vivo expression.