Characterization of the spatial distribution and abundance of proteins and mRNAs with morphological context within tissues enables a better understanding of biological systems in many research areas, including immunology, oncology and neuropathology. Analysis of samples across multiple tumor types and diseases has revealed novel spatially distinct protein and mRNA candidate biomarkers. However, it has proven difficult to perform such studies in a highly multiplexed manner at a throughput scale that is required for translational research programs. To address this unmet need, we have developed a novel platform that can perform high-plex analysis of proteins or mRNAs on a single FFPE section from distinct tissue spatial regions (GeoMx® Digital Spatial Profiler, DSP). Integrating the GeoMx DSP with either the NanoString nCounter or high-throughput sequencing, hundreds or thousands of spatially resolved analytes can be measured. To enhance both throughput and reproducibility, we have developed automated sample processing workflows for both protein and RNA on Leica Biosystem's BOND RX system. In this webinar, we will show you how the integrated workflows of the Leica BOND RX and the DSP can advance your translational research.

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There are several significant considerations to keep in mind when developing successful and reproducible staining assays. Choosing the appropriate detection method for tissue targets will allow researchers to produce the most compelling visual images to support their research goals. We will discuss some of the major factors that contribute to effective single and multiplex staining assays.

The Hematoxylin and Eosin stain (H&E) is the most widely used stain for histopathological analysis. Even though the H&E stain is a relatively simple stain to perform, there are a variety of factors that can interfere with the staining process. The maintenance of consistent, high-quality H&E stains is a fundamental requirement in any histopathology related project. Troubleshooting H&E stains can be difficult and frustrating. Our objective is to highlight some of the most common mistakes when choosing staining reagents and creating protocols. By minimizing them, we can deliver high quality slides in the shortest possible time to improve the outcome of translational research projects.

Since the 19th century, the practice of tissue processing has remained largely unchanged, resulting in solidified, paraffin-embedded tissue blocks for sectioning. As staining procedures have advanced and immunohistochemistry is becoming more widely used, high-quality tissue sections are paramount for accurate interpretation of disease processes. At each processing stage, many variables require consideration to eliminate artifacts and preserve tissue morphology to ensure high-quality sectioning. This talk will review basic strategies to develop best laboratory practices and protocols from the beginning of the histology workflow, which results in good quality sections to minimize the need for repeat downstream testing.

Once the tissue has been processed well, getting good sections placed onto the glass slide should be easier; however, this is not always the case, with some cases proving challenging to cut. In this section of the talk, we will review the basics of embedding and sectioning, including paraffin types, molds, blades, and slides, as well as a discussion of artifacts. We will then present the “tricks of the trade” or “histology hacks” that almost every seasoned histologist has kept secret until now.