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OCT 17, 2024 5:00 AM PDT

How the pros build a better qPCR assay

SPONSORED BY: LGC Biosearch Technologies

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Reading through huge amounts of published qPCR data to select the best experimental framework for your objectives requires a significant amount of time, expertise and resources. Wouldn’t it be great to have a guide packed full of pro tips on how to build and troubleshoot qPCR assays, right at your fingertips? Level up your qPCR assay with advice from the pros. This guide provides you with specific considerations for assay optimisation and validation based on practical application of the Minimum Information for Publication of Quantitative Real-Time (RT) PCR Experiments (MIQE) guidelines.

With this guide you will:

Learn how to optimise your assay by evaluating and adjusting design elements to improve performance
Assess the key performance parameters that impact the assay’s efficiency, technical dynamic range and reproducibility
Receive recommendations for precise characterisation of assay sensitivity, specificity and precision
Leverage a combination of normalisation methods to address challenges at every step of the protocol
Get our experts' tips on data analysis in qPCR

About the Sponsor

LGC Biosearch Technologies

Biosearch Technologies is the comprehensive genomics portfolio from LGC, providing products and services for genomic analysis that support mission critical applications for global customers in agrigenomics and human healthcare.

The portfolio offers integrated tools and technologies to accelerate sample preparation, amplification, cloning and expression, next generation sequencing (NGS), custom oligonucleotide synthesis, nucleic acid chemistry reagents, and oligo therapeutics.

Whether through sbeadex™ DNA purification, oligos and reagents (KASP™, BHQplus™ Probes, BHQ™ Probe Master Mix), or proprietary sample prep and PCR instrumentation (oKtopure™, SNPline™, Nexar™ and IntelliQube™), we enable our customers from discovery to production-scale testing.

Biosearch Technologies is a leader in the agricultural genomics market and is increasingly adopted by researchers, with over 2,000 scientific papers citing KASP assays. Key applications include validation and screening of newly discovered SNPs in Marker-Assisted Selection and Marker-Assisted Breeding programmes for both plants and livestock.

Globally, our cGMP manufacturing and ISO 13485-compliant facilities support supply chain continuity, complementing our customers' product development pipelines through our robust research grade and cGMP oligonucleotide manufacturing that achieves consistent scale up. For molecular diagnostics, Biosearch Technologies has proven expertise in NGS, isothermal, qPCR-based applications where our tools and technologies such as BHQ probes and proprietary PCR platforms are frequently used in LDTs, ASRs and in vitro diagnostics. Through our recently acquired Lucigen portfolio, we also offer custom and bulk enzymes and buffers as critical components for molecular diagnostics.

Sales offices are located in China, Germany, Singapore, UK, and US. Sales representatives are available throughout Europe, Latin America, and Asia Pacific, with distributors in other key territories. Visit www.biosearchtech.com