Unlocking Precision: The BD PAXgene® Blood RNA tubes, as part of the Cell and Biomarker Preservation portfolio for research tubes, are an essential component of the PAXgene® Blood RNA System. This tube is part of a comprehensive system that integrates whole blood collection, intracellular RNA stabilization, and RNA purification. This is essential due to RNA’s high susceptibility to degradation, especially in whole blood samples. By preserving RNA integrity, researchers can obtain accurate and reliable gene expression data. Containing a proprietary reagent that stabilizes intracellular RNA upon collection, this stabilization lasts for up to three days at room temperature, or five days at 2-8 degrees Celsius.
The PAXgene® Blood RNA System is the first in-vitro diagnostic (IVD) marketed product designed specifically for blood collection, storage, and transportation. It enables subsequent isolation and purification of intracellular RNA from whole blood for RT-PCR used in molecular diagnostic testing. Some additional roles in various molecular diagnostics and gene expression studies include disease biomarker discovery, pharmacogenomics, cardiovascular research, neurological disorders and infectious diseases. For example, a 2007 study investigated the use of PAXgene® Blood RNA Tubes for RNA stabilization and subsequent gene expression analysis in immunogenomic studies. The study demonstrated that RNA can be reliably detected and quantified in peripheral blood collected into the RNA tubes, while comparing RNA levels between patients with various conditions including diabetic retinopathy, and thyroid cancer. This study emphasized the successful downstream application of PAXgene® Blood RNA Tubes in immunogenomic investigations and provides valuable insight into how these tubes contribute to various research by ensuring accurate and reliable results for molecular diagnostic testing.
Collecting blood for RNA stabilization and isolation can be challenging, as RNA transcript levels exhibit rabid and substantial changes due to factors such as post-phlebotomy gene induction, cell death and enzymatic RNA degradation. These fluctuations can ultimately result in greater variability in RNA quantity and yield. An effective solution has been the use and application of the RNA PAXgene® Blood RNA system. The PAXgene® Blood RNA Tubes have been instrumental in advancing research across various fields and provide a standardized and reliable method for collecting and preserving blood samples.
BD PAXgene® Blood RNA Tubes are a remarkable innovation in the field of molecular diagnostics, providing a reliable solution for RNA stabilization and subsequent molecular diagnostic testing. From immediate stabilization of intracellular RNA upon collection from whole blood to accurate gene expression data these tubes offer a high-quality solution for preserving valuable samples.
Visit BD’s Cell and Biomarker Preservation website today for more information.
For any questions regarding BD Blood Collection or the PAXgene portfolio, please email cbp-can@bd.com
References:
Carrol, E. D., Salway, F., Pepper, S. D., Saunders, E., Mankhambo, L. A., Ollier, W. E., Hart, C. A., & Day, P. (2007). Successful downstream application of the Paxgene Blood RNA system from small blood samples in pediatric patients for quantitative PCR analysis. BMC Immunology
PreAnalytiX. (n.d.). PAXgene Blood RNA Tube (IVD). For collection of whole blood samples for molecular diagnostic testing. Coupled with the PAXgene Blood RNA Kit (IVD), constitutes the PAXgene Blood RNA System. Single tube for collection, storage, and transport: https://www.preanalytix.com/knowledge/blood/rna/rna-application/CA?cHash=7c0ee5277373dcf3
