Follow
Western Blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used in molecular biology, immunogenetics and other molecular biology disciplines to detect specific proteins in a sample of tissue homogenate or extract. In brief, the sample undergoes protein denaturation, followed by gel electrophoresis. Synthetic or animal-derived antibodies are created that recognise and bind to a specific target protein, known as the primary antibody. The electrophoresis membrane is washed in a solution containing the primary antibody, before excess antibody is washed off. A secondary antibody is added which recognises and binds to the primary antibody. The secondary antibody is visualised through various methods such as staining, immunofluorescence, and radioactivity, allowing indirect detection of the specific target protein.
SEP 23, 2020 | 3:00 PM
For more than a century, breakthroughs in biological sciences have relied on the ability to study cells outside of respective organisms. While majority of cell culturing is still performed u...
SEP 22, 2020 | 11:00 AM
DATE: August 22, 2020 TIME: 9:00am PDT The western blotting technique that began as a means of detecting the protein of interest in a complex sample that may contain numerous other proteins...
SEP 03, 2020 | 9:00 AM
DATE: September 3, 2020 TIME: 09:00am PT, 12:00pm ET xxx Learning Objectives: xxx Webinars will be available for unlimited on-demand viewing after live event. LabRoots is approved as a provi...
JUL 08, 2020 | 8:00 AM
DATE: July 8, 2020 TIME: 8:00am PDT In this webinar, discover how targeted protein degradation is being used to drive drug discovery. Hosted by Gary Allenby, Chief Executive Officer of Aurel...
JUN 18, 2020 | 9:00 AM
DATE: June 18, 2020 TIME: 9:00am PDT, 12:00pm EDT Despite the success of immunotherapy against several malignancies including melanoma and lung adenocarcinoma, glioblastoma remains the excep...
JUN 03, 2020 | 2:00 PM
While providing indispensable insight into immune processes, the live cell imaging of immune cells poses unique challenges due to their non-adherent nature. Suspension cells such as immune c...
APR 28, 2020 | 10:00 AM
DATE: April 28, 2020 TIME: 10:00am PT All cells release extracellular vesicles (EVs) that can carry molecular cargo to other cells to affect their function. EVs are promising as potential bi...
APR 14, 2020 | 9:00 AM
DATE: April 14, 2020 TIME: 9:00am PDT, 12:00pm EDT Besides being Gibco Cell Culture Heroes, what do Vivek, Sandra, Daisy, Ameet and Kristine all have in common? They are all stuck at home fo...
MAR 27, 2020 | 9:00 AM
DATE: March 27, 2020 TIME: 9:00am PDT, 12:00pm EDT Osteoporosis causes a decrease in bone density, along with deterioration of the bone’s microarchitecture at a faster rate than normal...
JAN 30, 2020 | 9:00 AM
DATE: January 30, 2020 TIME: 9:00am PST, 12:00pm EST Recent advances in in vitro 3D cellular culture technologies, such as organoids, rapidly developed and established novel, more physiologi...
DEC 17, 2019 | 9:00 AM
DATE: December 17, 2019TIME: 9:00am PST, 12:00pm EST Purpose: Liver x receptors (LXRs) are hypothesized to serve as a link between lipid metabolism and inflammation by prom...
DEC 04, 2019 | 7:00 AM
DATE: December 4, 2019TIME: 7:00am PST, 10:00am EST, 4:00pm CET Do you know how it feels when you just quickly want to redo an experiment that your colleague did, or an experim...
NOV 18, 2019 | 7:00 AM
DATE: November 18, 2019TIME: 7:00am PST, 11:00am EST, 4:00pm CEWT How often do you pipette in your cell culture lab every day? Usually, we do it so often that we tend stop th...
NOV 05, 2019 | 7:00 AM
DATE: November 5, 2019 TIME: 7:00am PST, 9:00am CSTDetection of low-abundance proteins and/or detection of proteins from precious samples can be a major challenge when performing wes...
SEP 24, 2019 | 9:00 AM
DATE: September 24, 2019TIME: 9:00am PDTThis webinar will cover the basics of the Milo Single-Cell Western blot (SCW) and RNAScope single-molecule fluorescence RNA In situ hybridizati...
SEP 05, 2019 | 7:00 AM
DATE: September 5, 2019TIME: 7:00am PT, 10:00am ET, 4:00pm CEST PCR (Polymerase Chain Reaction) has gone through a massive evolution since its development in 1983. Besides it...
JUN 05, 2019 | 5:00 PM
DATE: June 5, 2019TIME: 8:00am PDT, 11:00am EDT, 5:00pm CEST Eukaryotic cell cultures respond to the most subtle influence. Apart from the risk of contamination, minimal chan...
MAY 23, 2019 | 9:00 AM
DATE: May 23, 2019TIME: 9:00am PDT, 12:00pm EDT Although mesenchymal stem/stromal cells (MSCs) chondrogenic differentiation has been thoroughly investigated...
MAY 16, 2019 | 4:00 PM
DATE: May 16, 2019TIME: 7:00am PDT, 10:00am EDT, 4:00pm CEST The emergence of NGS is revolutionizing the microbiological sciences and transforming medicine. Deep sequencing has...
APR 09, 2019 | 9:00 AM
DATE: April 9, 2019TIME: 9:00 AM PDTWe will provide a general introduction to the Single-Cell Western Technology (SCW) and then present data from 2 ongoing projects from the Col...
DEC 13, 2018 | 9:00 AM
DATE: December 13 15,2018TIME: 09:00am PST, 12:00pm EST Breast cancers are classified into three main subtypes according to their receptor status: estrogen receptor-positive...
OCT 11, 2018 | 1:30 PM
As the most common female malignancy, breast cancer is the most likely reason that a woman will die of cancer around the world. Breast cancer mortality has dropped in the U.S. by 35% since 19...
OCT 11, 2018 | 12:00 PM
Lung cancer is the leading cause of cancer-related mortality worldwide. Large-scale sequencing studies have revealed the complex genomic landscape of NSCLC and genomic differences between lun...
APR 28, 2020 | 10:00 AM
DATE: April 28, 2020 TIME: 10:00am PT All cells release extracellular vesicles (EVs) that can carry molecular cargo to other cells to affect their function. EVs are promising as potential bi...
APR 14, 2020 | 9:00 AM
DATE: April 14, 2020 TIME: 9:00am PDT, 12:00pm EDT Besides being Gibco Cell Culture Heroes, what do Vivek, Sandra, Daisy, Ameet and Kristine all have in common? They are all stuck at home fo...
MAR 27, 2020 | 9:00 AM
DATE: March 27, 2020 TIME: 9:00am PDT, 12:00pm EDT Osteoporosis causes a decrease in bone density, along with deterioration of the bone’s microarchitecture at a faster rate than normal...
JAN 30, 2020 | 9:00 AM
DATE: January 30, 2020 TIME: 9:00am PST, 12:00pm EST Recent advances in in vitro 3D cellular culture technologies, such as organoids, rapidly developed and established novel, more physiologi...
DEC 17, 2019 | 9:00 AM
DATE: December 17, 2019TIME: 9:00am PST, 12:00pm EST Purpose: Liver x receptors (LXRs) are hypothesized to serve as a link between lipid metabolism and inflammation by prom...
DEC 04, 2019 | 7:00 AM
DATE: December 4, 2019TIME: 7:00am PST, 10:00am EST, 4:00pm CET Do you know how it feels when you just quickly want to redo an experiment that your colleague did, or an experim...
NOV 18, 2019 | 7:00 AM
DATE: November 18, 2019TIME: 7:00am PST, 11:00am EST, 4:00pm CEWT How often do you pipette in your cell culture lab every day? Usually, we do it so often that we tend stop th...
NOV 05, 2019 | 7:00 AM
DATE: November 5, 2019 TIME: 7:00am PST, 9:00am CSTDetection of low-abundance proteins and/or detection of proteins from precious samples can be a major challenge when performing wes...
SEP 24, 2019 | 9:00 AM
DATE: September 24, 2019TIME: 9:00am PDTThis webinar will cover the basics of the Milo Single-Cell Western blot (SCW) and RNAScope single-molecule fluorescence RNA In situ hybridizati...
SEP 05, 2019 | 7:00 AM
DATE: September 5, 2019TIME: 7:00am PT, 10:00am ET, 4:00pm CEST PCR (Polymerase Chain Reaction) has gone through a massive evolution since its development in 1983. Besides it...
JUN 05, 2019 | 5:00 PM
DATE: June 5, 2019TIME: 8:00am PDT, 11:00am EDT, 5:00pm CEST Eukaryotic cell cultures respond to the most subtle influence. Apart from the risk of contamination, minimal chan...
MAY 23, 2019 | 9:00 AM
DATE: May 23, 2019TIME: 9:00am PDT, 12:00pm EDT Although mesenchymal stem/stromal cells (MSCs) chondrogenic differentiation has been thoroughly investigated...
MAY 16, 2019 | 4:00 PM
DATE: May 16, 2019TIME: 7:00am PDT, 10:00am EDT, 4:00pm CEST The emergence of NGS is revolutionizing the microbiological sciences and transforming medicine. Deep sequencing has...
APR 09, 2019 | 9:00 AM
DATE: April 9, 2019TIME: 9:00 AM PDTWe will provide a general introduction to the Single-Cell Western Technology (SCW) and then present data from 2 ongoing projects from the Col...
DEC 13, 2018 | 9:00 AM
DATE: December 13 15,2018TIME: 09:00am PST, 12:00pm EST Breast cancers are classified into three main subtypes according to their receptor status: estrogen receptor-positive...
OCT 11, 2018 | 1:30 PM
As the most common female malignancy, breast cancer is the most likely reason that a woman will die of cancer around the world. Breast cancer mortality has dropped in the U.S. by 35% since 19...
OCT 11, 2018 | 12:00 PM
Lung cancer is the leading cause of cancer-related mortality worldwide. Large-scale sequencing studies have revealed the complex genomic landscape of NSCLC and genomic differences between lun...