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Western Blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used in molecular biology, immunogenetics and other molecular biology disciplines to detect specific proteins in a sample of tissue homogenate or extract. In brief, the sample undergoes protein denaturation, followed by gel electrophoresis. Synthetic or animal-derived antibodies are created that recognise and bind to a specific target protein, known as the primary antibody. The electrophoresis membrane is washed in a solution containing the primary antibody, before excess antibody is washed off. A secondary antibody is added which recognises and binds to the primary antibody. The secondary antibody is visualised through various methods such as staining, immunofluorescence, and radioactivity, allowing indirect detection of the specific target protein.
What's Popular in Western Blot
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FEB 05, 2024Immunology
Introduction Proteins are essential molecules in the human body that construct the building blocks of life. Long chains ...Sponsored By: MilliporeSigmaOCT 17, 2023Cancer
Cutaneous squamous cell carcinoma (cSCC) is a common form of skin cancer often caused by chronic exposure to ultraviolet ...Written By: Greta AnneAUG 09, 2023Genetics & Genomics
There are about 20,000 genes that encode for protein in the human genome, and we don't know much about many of them. ...Written By: Carmen LeitchJAN 03, 2023Genetics & Genomics
Researchers can easily assess what genes are being expressed in a tissue by using a method like RT-PCR that precisely an ...Written By: Carmen LeitchSEP 06, 2022Microbiology
Introduction Monkeypox virus (MPXV) is an old virus that has recently grabbed attention because of its emergence in unco ...Sponsored By: Sino Biological
FEB 05, 2024
Immunology
Introduction Proteins are essential molecules in the human body that construct the building blocks of life. Long chains
...
Sponsored By:
MilliporeSigma
OCT 17, 2023
Cancer
Cutaneous squamous cell carcinoma (cSCC) is a common form of skin cancer often caused by chronic exposure to ultraviolet
...
Written By:
Greta Anne
AUG 09, 2023
Genetics & Genomics
There are about 20,000 genes that encode for protein in the human genome, and we don't know much about many of them.
...
Written By:
Carmen Leitch
JAN 03, 2023
Genetics & Genomics
Researchers can easily assess what genes are being expressed in a tissue by using a method like RT-PCR that precisely an
...
Written By:
Carmen Leitch
SEP 06, 2022
Microbiology
Introduction Monkeypox virus (MPXV) is an old virus that has recently grabbed attention because of its emergence in unco
...
Sponsored By:
Sino Biological
JUL 14, 2022
Clinical & Molecular DX
Magnetic beads (or superparamagnetic particles) can be used for the isolation and purification of cells, nucleic acids,
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FEB 11, 2022
Cannabis Sciences
According to a recent study published in Oncotarget, cannabis extracts may harbor the potential to eventually be tailore
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Written By:
Nadine Husami
DEC 01, 2021
Plants & Animals
Along with milk and peanut allergies, egg allergies are among the most common food allergies in the world. It’s es
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Written By:
Ryan Vingum
JAN 27, 2019
Neuroscience
A study out of John's Hopkins University published by Dr. Faith Dickerson, MD, and colleagues late last year have fo
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Written By:
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MAR 20, 2024 | 8:00 AM
Luminex and Thermo Fisher Scientific are excited to feature two presentations highlighting the advanced features of the Invitrogen™ Luminex® xMAP® INTELLIFLEX™ DR-SE inst...APR 11, 2023 | 8:00 AMC.E. CREDITS
Date: April 11, 2023 Time: 8:00am (PDT), 11:00am (EDT), 5:00pm (CEST) Duchenne muscular dystrophy is caused by mutations in the dystrophin encoding DMD gene that disrupt the reading frame. M...NOV 29, 2022 | 10:00 AM
Date: November 30, 2022 Time: 9:00am (PST), 12:00pm (EST), 6:00pm (CET) Western blotting is a commonly used analytical technique in protein analysis. However, the standard manual workflow fr...NOV 07, 2022 | 9:00 PMC.E. CREDITS
Date: November 8, 2022 Time: 1:00pm (SGT), 12:00pm (EST), 6:00am (CET) Antibodies are vital tools in many of the laboratory techniques that are used...NOV 10, 2021 | 3:00 PM
SARS-CoV-2 testing is now facing increasing challenges in the face of an ongoing pandemic with the emergence of new variants and waning vaccine immunity. Increasing evidence supports the cor...Speaker: Sean Taylor, MBA, PhD
MAR 20, 2024 | 8:00 AM
Luminex and Thermo Fisher Scientific are excited to feature two presentations highlighting the advanced features of the Invitrogen™ Luminex® xMAP® INTELLIFLEX™ DR-SE inst...
APR 11, 2023 | 8:00 AM
C.E. CREDITS
Date: April 11, 2023 Time: 8:00am (PDT), 11:00am (EDT), 5:00pm (CEST) Duchenne muscular dystrophy is caused by mutations in the dystrophin encoding DMD gene that disrupt the reading frame. M...
NOV 29, 2022 | 10:00 AM
Date: November 30, 2022 Time: 9:00am (PST), 12:00pm (EST), 6:00pm (CET) Western blotting is a commonly used analytical technique in protein analysis. However, the standard manual workflow fr...
NOV 07, 2022 | 9:00 PM
C.E. CREDITS
Date: November 8, 2022 Time: 1:00pm (SGT), 12:00pm (EST), 6:00am (CET) Antibodies are vital tools in many of the laboratory techniques that are used...
NOV 10, 2021 | 3:00 PM
SARS-CoV-2 testing is now facing increasing challenges in the face of an ongoing pandemic with the emergence of new variants and waning vaccine immunity. Increasing evidence supports the cor...
Speaker:
Sean Taylor, MBA, PhD
OCT 14, 2021 | 9:00 AM
Date: October 14, 2021 Time: 9:00am (PDT), 12:00pm (EDT), 5:00pm GMT Western blot analysis is a method widely used in the lab today because of its versatility in detecting and measuring spe...
Speaker:
Kelli Feather-Henigan
Presented at: Webinar series: Simple steps to publication-quality western blots
Sponsored By: Thermo Fisher Scientific
Sponsored By: Thermo Fisher Scientific
OCT 13, 2021 | 3:00 PM
SARS-CoV-2 testing is now facing increasing challenges in the face of an ongoing pandemic with the emergence of new variants and waning vaccine immunity. Increasing evidence supports the cor...
OCT 13, 2021 | 7:00 AM
Date: October 13, 2021 Time: 7:00am (PDT), 10:00am (EDT) Western blot analysis is a method widely used in the lab today because of its versatility in detecting and measuring specific target...
Speaker:
Caleb Shearrow
Presented at: Webinar series: Simple steps to publication-quality western blots
Sponsored By: Thermo Fisher Scientific
Sponsored By: Thermo Fisher Scientific
OCT 12, 2021 | 5:00 PM
Date: October 12, 2021 Time: 5:00pm (PDT), 8:00pm (EDT) 222 Learning Objectives 222 222 222 Webinars will be available for unlimited on-demand viewing after live event. LabRoots is approved...
Speaker:
Jordan Thompson, PhD
Presented at: Webinar series: Simple steps to publication-quality western blots
Sponsored By: Thermo Fisher Scientific
Sponsored By: Thermo Fisher Scientific
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SEP 08, 2021 | 6:00 AM PDT
Luminex's xMAP® Technology enables the evaluation of up to 500 analytes simultaneously in a single well, known as multiplexing. xMAP Technology uses color-coded microspheres as the substrate...
Western blot analysis is a method widely used in the lab today because of its versatility in detecting and measuring specific target proteins in a sample. However, in recent years the reprod...OCT 10, 2018 | 6:00 AM PDT
The 6th Annual Event in the Cancer Research & Oncology Week Virtual Event Series will take place October 2018....OCT 03, 2018 | 6:00 AM PDT
Labroots invites you to the 1st Annual Event in the CRISPR Virtual Event Series! With continued adoption and integration in research labs...SEP 17, 2018
Improve your protein research knowledge by joining our 7 Steps of Protein virtual eventThe 3rd annual 7 Steps of Protein virtual event is taking place around the world on Tuesday, Septemb...
SEP 08, 2021
| 6:00 AM PDT
Luminex's xMAP® Technology enables the evaluation of up to 500 analytes simultaneously in a single well, known as multiplexing. xMAP Technology uses color-coded microspheres as the substrate...
Western blot analysis is a method widely used in the lab today because of its versatility in detecting and measuring specific target proteins in a sample. However, in recent years the reprod...
OCT 10, 2018
| 6:00 AM PDT
The 6th Annual Event in the Cancer Research & Oncology Week Virtual Event Series will take place October 2018....
OCT 03, 2018
| 6:00 AM PDT
Labroots invites you to the 1st Annual Event in the CRISPR Virtual Event Series! With continued adoption and integration in research labs...
SEP 17, 2018
Improve your protein research knowledge by joining our 7 Steps of Protein virtual eventThe 3rd annual 7 Steps of Protein virtual event is taking place around the world on Tuesday, Septemb...
Experience the latest advances in protein expression at our 3rd annual virtual event In 2017, the virtual event had 2,400 international participants in over 80 countries! Th...
OCT 11, 2017
| 6:00 AM PDT
The 5th Annual Event in the Cancer Research & Oncology Week Virtual Event Series is now On-Demand!...
APR 10, 2017
| 6:00 AM PDT
The Protein and cell analysis education site is a free-access destination where you can learn more about applications and techniques related to protein gel chemistries, western detection, mas...
JAN 25, 2017
| 5:00 AM PST
The Abcam virtual exhibition is temporarily closed. It will re-open shortly. Please check back October 25th. All webinars will be available for viewing at that time.Get the su...