Fluorescence in situ hybridization - aka FISH is defined as a molecular cytogenetic technique that uses fluorescent probes that bind to only those parts of a nucleic acid sequence with a high degree of sequence complementarity. Developed in the early 1980's, biomedical researchers developed it to localize and detect the absence or presence of specific DNA sequences on chromosomes. Fluorescence microscopy can be utilized to find out where the fluorescent probe is bound to the chromosomes. FISH is often used for finding specific features in DNA for use in genetic counseling, medicine, and species identification. FISH can also be used to detect and localize specific Ribonucleic acid or RNA targets in tissue samples, circulating tumor cells, and cells, assisting in defining the spatial-temporal patterns of gene expression within tissues and cells.
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Date: November 19, 2020 Time: 12:00am (PDT), 9:00am (CET), 4:00pm (SGT) We present split-FISH, a multiplexed fluorescence in situ hybridization method that leverages a split-probe design to...
In Pacific Northwest watersheds several species of Pacific salmonid fishes are hosts for the rhabdovirus infectious hematopoietic necrosis virus (IHNV). In this multi-host ecosystem speciali...
Microbial biofilms form on all aquatic surfaces and can harbor pathogenic bacteria. In the aquaculture industry, Flavobacteria species can cause serious diseases and lead to high mortality....
Next-generation sequencing (NGS) has become the most effective tool in the practice of clinical oncology. The technology allows for rapid detection of variants in DNA/RNA simultaneously in a...
Next-generation sequencing (NGS) has become the most effective tool in the practice of clinical oncology. The technology allows for rapid detection of variants in DNA/RNA simultaneously in a...
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