Biology: the study of living organisms, divided into many specialized fields that cover their morphology, physiology, anatomy, behavior, origin, and distribution. This includes plants, animals, and other qualities of organisms or class of organisms.
This lecture will encompass the learning experience I have had over my 14,000 RALP cases. We will present an evidence based prospective data collection and analysis of RALP outcomes. We will...
Immune therapy approaches are being used in many different solid cancers today including clear cell renal carcinoma (mccRCC) and metastatic prostate adenocarcinoma. Over the past few years i...
Activating mutations in PIK3CA are frequent in human breast cancer, and phosphoinositide 3-kinase alpha (PI3Kα) inhibitors have been approved for therapy. To characterize determinants...
Date: October 10, 2020 Time: 10:00am (PDT), 1:00pm (EDT) Rodent bile collection studies can be challenging. Not only do they require a technically difficult surgery, but there is also a lear...
RNA plays important and diverse roles in biology, but molecular tools to manipulate and measure RNA are limited. We demonstrate that RNA-targeting CRISPR effector Cas13 can be engineered for...
The CRISPR/Cas genome editing system has revolutionized nearly every aspect of the life science industry. Until recently, the most used formats for this technology have been plasmids, mRNA,...
CRISPR-based genome editing has accelerated biological research and holds great potential for studying and treating human diseases. The CRISPR-Cas9 system requires a Cas9 nuclease and a guid...
While transformative, first-generation CRISPR technologies remain limited across multiple important dimensions including scalability, editing efficiency, types of modifications available, an...
Genome Engineering allows the easy manipulation of genomes down to the nucleotide level. Targeted deep sequencing enables the detection and quantification of low-frequency editing events. Ho...
Genome editing with an expanded CRISPR-Cas tool set increases the opportunity to make intentional, targeted changes in the genome. Furthermore, improved specificity of genome editing and abi...
Combinatorial inhibition of effector and feedback pathways is a promising treatment strategy for KRAS mutant cancers. However, the particular pathways that should be targeted to optimize the...
Learning Objectives: 1. Using an optics- and label-free approach to CRISPR research 2. Explain current approaches to selecting the most robust gRNA for CRISPR assays 3. The opportunities tha...
The adenosine analogue remdesivir has emerged as a front-line antiviral treatment for SARS-CoV-2, with preliminary evidence that it reduces the duration and severity of illness. Prior clinic...
CRISPR experiments are a powerful tool which are easy to carry out, however it is more difficult to determine the outcome of these experiments, and to ensure that only the desired targets we...
An extensively debated concern about CRISPR-Cas9 genome editing is that unspecific guide RNA (gRNA) binding may induce off-target mutations. However, accurate prediction of CRISPR-Cas9 off-t...
A repeat expansion in the C9orf72-SMCR8 complex subunit (C9orf72) represents the most common genetic cause of two fatal neurodegenerative diseases: frontotemporal dementia (FTD) and amyotrop...
Cas9 based therapeutics have the potential to revolutionize the treatment of genetic diseases. However, safe and effective methods for delivering Cas9 protein, gRNA and donor DNA need to be...
Date: September 28, 2020 Time: 5:00pm (PDT), 8:00am (HKT), 9:00am (JST) Although there have been dramatic advances in the field of single-cell sequencing, current methods cannot be applied t...
There are many methods of nucleic acid isolation. Each technology offers different approaches of purification of the template. However, it is important to control carry over of “proces...
For more than a century, breakthroughs in biological sciences have relied on the ability to study cells outside of respective organisms. While majority of cell culturing is still performed u...
Extracellular vesicles (EVs) are lipid bilayer-delimited pieces of cells that are released from the plasma membrane as "ectosomes" and from the endosomal system as "exosomes.&...
Extracellular ligands bind to receptors on the cell surface leading to receptor internalization. Once internalized into small vesicles, the vesicles fuse with an organelle known as the sorti...
Intestinal organoids are self-organizing, 3D structures derived from either pluripotent stem cells or from primary tissues with the abiltiy to recapitulate some of the spatial architecture a...
This lecture will encompass the learning experience I have had over my 14,000 RALP cases. We will present an evidence based prospective data collection and analysis of RALP outcomes. We will...
Immune therapy approaches are being used in many different solid cancers today including clear cell renal carcinoma (mccRCC) and metastatic prostate adenocarcinoma. Over the past few years i...
Activating mutations in PIK3CA are frequent in human breast cancer, and phosphoinositide 3-kinase alpha (PI3Kα) inhibitors have been approved for therapy. To characterize determinants...
Date: October 10, 2020 Time: 10:00am (PDT), 1:00pm (EDT) Rodent bile collection studies can be challenging. Not only do they require a technically difficult surgery, but there is also a lear...
RNA plays important and diverse roles in biology, but molecular tools to manipulate and measure RNA are limited. We demonstrate that RNA-targeting CRISPR effector Cas13 can be engineered for...
The CRISPR/Cas genome editing system has revolutionized nearly every aspect of the life science industry. Until recently, the most used formats for this technology have been plasmids, mRNA,...
CRISPR-based genome editing has accelerated biological research and holds great potential for studying and treating human diseases. The CRISPR-Cas9 system requires a Cas9 nuclease and a guid...
While transformative, first-generation CRISPR technologies remain limited across multiple important dimensions including scalability, editing efficiency, types of modifications available, an...
Genome Engineering allows the easy manipulation of genomes down to the nucleotide level. Targeted deep sequencing enables the detection and quantification of low-frequency editing events. Ho...
Genome editing with an expanded CRISPR-Cas tool set increases the opportunity to make intentional, targeted changes in the genome. Furthermore, improved specificity of genome editing and abi...
Combinatorial inhibition of effector and feedback pathways is a promising treatment strategy for KRAS mutant cancers. However, the particular pathways that should be targeted to optimize the...
Learning Objectives: 1. Using an optics- and label-free approach to CRISPR research 2. Explain current approaches to selecting the most robust gRNA for CRISPR assays 3. The opportunities tha...
The adenosine analogue remdesivir has emerged as a front-line antiviral treatment for SARS-CoV-2, with preliminary evidence that it reduces the duration and severity of illness. Prior clinic...
CRISPR experiments are a powerful tool which are easy to carry out, however it is more difficult to determine the outcome of these experiments, and to ensure that only the desired targets we...
An extensively debated concern about CRISPR-Cas9 genome editing is that unspecific guide RNA (gRNA) binding may induce off-target mutations. However, accurate prediction of CRISPR-Cas9 off-t...
A repeat expansion in the C9orf72-SMCR8 complex subunit (C9orf72) represents the most common genetic cause of two fatal neurodegenerative diseases: frontotemporal dementia (FTD) and amyotrop...
Cas9 based therapeutics have the potential to revolutionize the treatment of genetic diseases. However, safe and effective methods for delivering Cas9 protein, gRNA and donor DNA need to be...
Date: September 28, 2020 Time: 5:00pm (PDT), 8:00am (HKT), 9:00am (JST) Although there have been dramatic advances in the field of single-cell sequencing, current methods cannot be applied t...
There are many methods of nucleic acid isolation. Each technology offers different approaches of purification of the template. However, it is important to control carry over of “proces...
For more than a century, breakthroughs in biological sciences have relied on the ability to study cells outside of respective organisms. While majority of cell culturing is still performed u...
Extracellular vesicles (EVs) are lipid bilayer-delimited pieces of cells that are released from the plasma membrane as "ectosomes" and from the endosomal system as "exosomes.&...
Extracellular ligands bind to receptors on the cell surface leading to receptor internalization. Once internalized into small vesicles, the vesicles fuse with an organelle known as the sorti...
Intestinal organoids are self-organizing, 3D structures derived from either pluripotent stem cells or from primary tissues with the abiltiy to recapitulate some of the spatial architecture a...
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