mouse monoclonal IgG1; Smad4 Antibody (B-8) is an IgG1 κ mouse monoclonal Smad4 antibody that detects the Smad4 protein of mouse, rat and human origin by WB, IP, IF, IHC(P), FCM and ELISA. Smad4 Antibody (B-8) is available as both the non-conjugated anti-Smad4 antibody form, as well as multiple conjugated forms of anti-Smad4 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Smad4 (also designated DPC4) is a member of the Smad family of proteins, which are the mammalian homologs of the Drosophila Mothers against dpp (Mad) . Smad proteins have been implicated as downstream effectors of TGFβ/BMP signaling. Smad1 (also designated Madr1 or JV4-1), Smad5 and mammalian Smad8 (also designated Smad9 or MADH6) are effectors of BMP2 and BMP4 function while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFβ and activin-mediated growth modulation. The Smad4 Antibody (B-8) shows specificity for Smad4, which has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGFβ signaling by interfering with TGFβ-mediated phosphorylation of other Smad family members.