mouse monoclonal IgG1; p-Thr Antibody (H-2) is an IgG1 κ mouse monoclonal p-Thr antibody (also designated p-Thr antibody or phosphorylated threonine antibody) that detects phosphothreonine-containing proteins of broad origin by WB, IP, IF, IHC(P), FCM and ELISA. p-Thr Antibody (H-2) is available as the non-conjugated anti-p-Thr antibody form, as well as multiple conjugated forms of anti-p-Thr antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Protein kinases catalyze the phosphorylation of serine, threonine or tyrosine residues in target substrates, providing a mechanism of control for myriad cellular signaling pathways. Threonine phosphorylation (also designated phosphorylated threonine antibody, phospho threonine antibody, or p-threonine antibody) plays a role in the activation of ERK and JNK MAP kinases, which are dually phosphorylated on tyrosine and threonine residues by MEK family kinases (1,2). Several families of kinases phosphorylate both serine and threonine residues in target substrates, including the Raf, Rsk, ROCK, PAK, Ak, and PKC families of protein kinases (3-8). Antibodies to phosphothreonine may be used for the characterization of proteins with phosphorylated threonine residues, and for the elucidation of cellular pathways involving threonine phosphorylation.