mouse monoclonal IgG1; ENOPH1 Antibody (H-10) is an IgG1 κ mouse monoclonal ENOPH1 antibody (also designated ENOPH1 antibody) that detects the ENOPH1 protein of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. ENOPH1 Antibody (H-10) is available as both the non-conjugated anti-ENOPH1 antibody form, as well as multiple conjugated forms of anti-ENOPH1 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. ENOPH1 (enolase-phosphatase 1), also known as E1, MASA or MST145, is a member of the MasA family of the HAD (halo-acid dehalogenase)-like hydrolase superfamily. Existing as a monomer and binding magnesium as a cofactor, ENOPH1 is a bifunctional enzyme, exhibiting both phosphatase and atypical enolase activities. ENOPH1 plays an important role in the ubiquitous methionine salvage pathway, a biochemical pathway found in all organisms that regulates methionine levels in the cell (also known as the Yang cycle in plants). More specifically, ENOPH1 catalyzes the continuous enolization and dephosphorylation of 2,3-diketo-5-methylthio-1-phosphopentane to yield the aci-reductone metabolite 1,2-dihydroxy-3-keto-5-methylthiopentene. Due to alternative splicing events, two isoforms exist for ENOPH1.