mouse monoclonal IgG1; p-Cofilin 1 Antibody (H-2) is an IgG1 κ mouse monoclonal p-Cofilin 1 antibody (also designated p-Cofilin 1 antibody or Cofilin antibody) that detects Ser 3 phosphorylated Cofilin 1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. p-Cofilin 1 Antibody (H-2) is available as the non-conjugated anti-p-Cofilin 1 antibody form, as well as multiple conjugated forms of anti-p-Cofilin 1 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. LIM-kinase 1 (LIMK-1) is a serine/threonine kinase containing LIM and PDZ domains. LIMK1 phosphorylates cofilin at Serine 3 both in vitro and in vivo. Cofilin is an actin-depolymerizing factor and regulates actin cytoskeletal reorganization. Phosphorylation of cofilin on serine 3 is known to block these activities. Phosphorylation of ADF/cofilin proteins by LIMK1 or other enzymes will tend to stabilize actin filaments by inhibiting the ability of these proteins to sever and depolymerize older actin filaments that have hydrolyzed their bound ATP and dissociated the phosphate. The rapid turnover of actin filaments and the tertiary meshwork formation are regulated by a variety of actin-binding proteins. Cofilin, therefore, is a terminal effector of signaling cascades that evokes actin cytoskeletal rearrangement.