mouse monoclonal IgG1; HOTTL Antibody (F-8) is an IgG1 κ mouse monoclonal HOTTL antibody (also designated HOTTL antibody) that detects the HOTTL protein of human origin by WB, IP, IF and ELISA. HOTTL Antibody (F-8) is available as both the non-conjugated anti-HOTTL antibody form, as well as multiple conjugated forms of anti-HOTTL antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. HOTTL (Tubulin-tyrosine ligase-like protein 3) is a 352 amino acid protein encoded by the human gene TTLL3. HOTTL is believed to be part of the Tubulin-tyrosine ligase family and contains one TTL domain. The TTL domain is an approximately 350 amino acid module present in a family of eukaryotic proteins that could catalyze ligations of diverse amino acids to tubulins or other substrates. The TTL domain contains ATP-grasp-like motifs that correspond to the ATP/Mg(2+) binding site typical of enzymes with ATP-dependent carboxylate-amine/thiol ligase activity. Subject to several post-translational modifications, assembled microtubules are detyrosinated over time at the C-terminus of a-tubulin. After microtubular disassembly TTL proteins restore tyrosine residues back to the detyrosinated tubulin leading to a cycle of detyrosination/tyrosination. HOTTL also may play a role in tumor cell regulation.