Professor of Biochemistry; Affiliate, Morgridge Institute for Research University of Wisconsin-Madison (USA)
BIOGRAPHY
JUL 02, 2020 10:00 AM PDT
Hands-on Tutorial: Workflow Advances for Whole Cell Correlative Cryo-Microscopy
Speakers
-
Jae E. Yang, Ph.D.
Postdoctoral Research Associate, Wright Laboratory, University of Wisconsin-Madison (USA)BIOGRAPHY -
Bryan S. Sibert, Ph.D.
Postdoctoral Research Associate, Wright Laboratory, University of Wisconsin-Madison (USA)BIOGRAPHY -
Joseph Y. Kim
Graduate Student, Wright Laboratory University of Wisconsin-Madison (USA)BIOGRAPHY
Event Date & Time
DATE: July 2, 2020
TIME: 10:00am PT, 1:00pm ET
Abstract
Correlative light and electron microscopy (CLEM) is used to accelerate biological discoveries. CLEM bridges between different microscopes and imaging modalities to study systems in 4D, effectively spanning gaps between cell and structural biology. CLEM technologies are rapidly advancing in order to improve biological understanding, experimental reproducibility, and automation. In this webinar, we will expand upon the basics of the technologies that were presented in the first webinar. We will describe successful step-by-step procedures for cell care, patterning substrates, culturing cells on substrates, and cryo-CLEM imaging. We will highlight validation steps and timing associated with stages throughout the process. We will also provide examples of biological systems that have been studied using these workflows.
Learning Objectives:
- Identify materials and instrumentation necessary for cryo-CLEM.
- Incorporate advanced micro-patterning methods into cryo-CLEM pipelines.
- Understand and use workflows for grid preparation, cell culture, and cryo-CLEM imaging.
Webinars will be available for unlimited on-demand viewing after live event.
LabRoots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.
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