Hands-on Tutorial: Workflow Advances for Whole Cell Correlative Cryo-Microscopy

Correlative light and electron microscopy (CLEM) is used to accelerate biological discoveries. CLEM bridges between different microscopes and imaging modalities to study systems in 4D, effectively spanning gaps between cell and structural biology. CLEM technologies are rapidly advancing in order to improve biological understanding, experimental reproducibility, and automation. In this webinar, we will expand upon the basics of the technologies that were presented in the first webinar. We will describe successful step-by-step procedures for cell care, patterning substrates, culturing cells on substrates, and cryo-CLEM imaging. We will highlight validation steps and timing associated with stages throughout the process. We will also provide examples of biological systems that have been studied using these workflows.

 

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