Application Scientist, Quantitative Proteomics, Thermo Fisher Scientific
BIOGRAPHY
SEP 15, 2021 7:40 AM PDT
Tips and Tricks in Instrument Method for TMT Quantitation Using Latest Version Instrument Control Software
Presented at:
Working in Tandem - 1st Annual TMT Symposium
Speaker
Abstract
Tandem Mass Tags (TMT) enables the high-throughput MS analysis for proteome quantitation. The ultimate goal in TMT MS experiment and method development has always been the improvement in quantified ID coverage, quantitation precision and accuracy. In this session, we will introduce how to acquire high quality TMT quantification data on Thermo Scientific Orbitrap Eclipse Tribrid mass spectrometer, Thermo Scientific Orbitrap Exploris 480 and 240 mass spectrometers. We will walk through the instrument methods using the latest version of instrument control software on each platform and will also cover the implementation of real-time search (RTS) and Thermo Fisher Scientific High-Field Asymmetric Waveform Ion Mobility Spectrometry (FAIMS) Pro interface in the data acquisition to further improve the quantitation performance of TMT workflows.
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