Staff Scientist, Protein Preparation & Analysis, Thermo Fisher Scientific
BIOGRAPHY
Date: May 23, 2024 | Time: 10am PDT, 1:00pm EDT
Know thy sample: Five methods to quantify your protein and when to use them
Speaker
Event Date & Time
Date: May 23, 2024
Time: 10am PDT, 1:00pm EDT
Abstract
Precise protein quantification is a crucial step in workflows involving protein extraction, isolation, and analysis. Accurate knowledge of the concentration of your protein sample is essential for proper setup and interpretation of experiments. It is also fundamental in understanding molecular interactions, signaling pathways, and pathological mechanisms, and in biomarker and drug discovery.
By attending this presentation, you will gain the foundational knowledge needed to choose the most suitable quantification method to answer your research questions.
Learning Objectives
- Describe how spectrophotometry, total protein assays, ELISA, western blotting, and mass spectrometry are used for protein quantification
- Understand which options are best for different scenarios based on your research objectives, sample compatibility, sensitivity, dynamic range, and ease of use
- Recognize common challenges for each method such as interference from contaminants or sample complexity
- Use tips and troubleshooting strategies to overcome common challenges
Webinars will be available for unlimited on-demand viewing after live event.
Labroots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.
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