Research Scientist, Life Sciences Solutions Group, Thermo Fisher Scientific
BIOGRAPHY
MAY 04, 2016 10:00 AM PDT
The ExpiCHO transient expression system: Optimization of monoclonal antibody protein A purification
Presented at:
2016 Gibco ExpressionWorld
Speaker
Abstract
The ExpiCHO transient expression system allows for the high titer production of a broad range of recombinant proteins. Due to the high titers observed for many proteins in the ExpiCHO system, and differences between CHO culture conditions, purification conditions may vary. In this presentation, protocols are presented for high efficiency, scalable, supernatant purification using Protein A chromatography resins. Differences in purification conditions for each culture condition are noted and product quality is discussed.
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