TCR-seq & BCR-seq Solutions: Applications to Clinical Research Projects

 

The application of next generation sequencing to interrogate immune repertoires and methods in which these highly complex datasets can be mined to examine immune regulation and/or identify potential biomarkers has evolved considerably over the years. With advances in sequencing technologies and development of comprehensive, intuitive analysis tools, NGS-based repertoire analysis has become more accessible to the broader research community. Thermo Fisher Scientific’s streamlined and user-friendly TCR- and BCR-seq laboratory and analysis pipelines enable just about anyone to incorporate this powerful tool into their research program.

 

In this discussion, we will present examples of how repertoire analysis can be used to answer different types of research questions. Highlight summaries of different studies leveraging TCR and BCR sequencing in human disease research to better understand mechanisms involved in inflammatory diseases will be presented. Methods of experimental design, sequencing, and analysis will be introduced for each, with examples of figures that can be generated with the user-friendly analysis portal.

 

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