Tape strip proteomic profiling for tumor-associated biomarker discovery

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Mycosis fungoides (MF), the most common type of cutaneous T-cell lymphoma, is characterized by malignant T-cell skin infiltration leading to inflammation and skin barrier defects. To effectively monitor disease activity, reproducible biomarkers in MF skin are essential. Skin tape-stripping is emerging as an easy and non-invasive method for tracking disease biomarkers. Tape stripping was performed on 39 patients with MF and 21 healthy volunteers at matched sites, after which protein levels in tape skin extracts were assessed using the proximity extension assay technology. Differential expression analysis revealed a total of 24 proteins upregulated in MF lesional skin, compared to MF non-lesional and healthy skin, spanning pathways of macrophage activation, angiogenesis, immune cell migration, malignant T-cell survival, apoptosis, and skin inflammation. Moreover, these differentially expressed proteins were associated with the MF microenvironment and certain MF clinical characteristics, supporting the use of tape strip proteomics for tracking skin disease activity and therapeutic response in MF.

 

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