The last decade has seen a significant shift in the way that mammalian cells are used in biomedical science. Researchers are increasingly turning from simple, reductionist recombinant cells to primary cells, stem-cell derived cells and advanced in vitro co-culture and organoid models for greater translation and wider application in their work. However, developing and using these different cell systems presents fresh challenges to the everyday researcher. These relate to workflows for cell handling, genetic manipulation, assay development, phenotypic analysis and clinical use in the case of cell therapy.

• Changing landscape of cellular research
• Overview of new requirements to monitor these advanced cell systems
• How continuous live-cell imaging and analysis are aligned to the new biological models