In situ single-cell transcriptomic imaging in formalin-fixed paraffin-embedded (FFPE) tissues with MERSCOPE

Formalin-fixed paraffin-embedded (FFPE) tissues are the most widely used clinical sample types in histology and molecular diagnosis. However, due to RNA degradation and protein crosslinking, FFPE samples are often not compatible with single-cell transcriptomic analysis. To accurately profile the gene expression in FFPE samples in situ, a spatial transcriptomics technique with high detection efficiency and single-molecule resolution is required. Vizgen’s in situ genomics platform MERSCOPE^TM is built on multiplexed error robust in situ hybridization (MERFISH) technology and enables the direct profiling of the spatial organization of intact tissue with subcellular resolution. In this webinar, Dr. He will present a workflow to perform in situ single-cell transcriptomic imaging in FFPE tissue sections with the MERSCOPE Platform. Using 3 independent custom gene panels (140, 300 and 500 gene panel) we will demonstrate MERFISH imaging in more than 15 FFPE sample types from mouse and human, including archival clinical samples.

 

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