Revolutionizing High Content Screening: From 2 hours to less than 10 minutes a plate

High content screening at speed requires compromise: sacrificing resolution, sample coverage or both. This has limited high content screening’s utility as a high throughput tool. In order to scale up a screen, several instruments may be required, introducing more variables. Araceli’s Endeavor high content screening system can image a 96, 384 or 1536 well microplate in under 10 minutes with full well coverage and sub-micron resolution, enabling high content screening at high throughput speeds without compromising resolution or coverage. This presentation details the technology underlying these improvements and its validation, as well as a novel quality control tool designed to assess large quantities of images at the level of content. After an overview of Endeavor’s capabilities on real-world assays, I’ll cover how Endeavor uniquely leverages several key technical innovations to achieve high content screening at scale. I'll describe how Endeavor’s proprietary parallelized optical paths and ground up design with no moving parts coalesce into an instrument that scanned forty-one 384-well plates at submicron resolution in an 8-hour workday. This produces >250,000 images: deciding which are worth further analysis is challenging. To that end, I’ll present a novel quality control algorithm to identify sample aberrations such as out of focus wells, dust, or pipet tip strikes. Overall, this presentation highlights the underlying technology that makes Endeavor the fastest high content screening platform available today, allowing you to scale up your assays without introducing errors or compromising quality.