Practical considerations for fluorescent cell staining and microscopy in cancer cell biology research

 

This seminar provides an overview of fluorescence microscopy in cancer research and other cell-based applications in the biosciences discovery workflow. In comparison with phase contrast and colorimetric cell analysis techniques, fluorescence imaging delivers robust, multi-parametric and scalable, cell-level analysis of large sample sizes in heterogenous populations with the benefit of spatial and temporal resolution. Following a basic primer on fluorescence imaging and cancer cell biology, a series of existing and newly developed tools for probing cellular structure, cell stress, signaling pathways and function will be reviewed in the context of cancer therapeutics discovery and mechanism of action research. Additional emphasis will be given to visualization of cell proliferation and cell surface target internalization, including tools for immuno-oncology research applications in both 2D and 3D models.

 

Learning Objectives