An overview of identification and quantification of TMT datasets using Proteome Discoverer 2.5

In this presentation, we will show how to use Proteome Discoverer 2.5 to obtain high quality results for TMT quantification data acquired with Thermo Scientific Orbitrap MS-based instruments. We will first provide an overview of the key features in Proteome Discoverer for peptide identification, quantification, and statistical analysis. We will then follow up with a few example datasets to show how these features can be applied.