Overcoming the high multiplexing barrier: 3D Spatial Omics in one go, powered by Leica Microsystems

The STELLARIS confocal platform offers significant advancements for high-multiplex strategies, integrating a tunable white light laser (WLL) with an excitation range from 440 nm to 790 nm and up to five highly sensitive, photon-counting spectral detectors. This configuration enables complete flexibility in detection from 410 nm to the near-infrared (NIR) spectrum, accommodating a broad array of fluorophores and optimizing their combinations for complex imaging tasks.

 

In this presentation, we will introduce the new SpectraPlex 3D high-multiplex solution for the STELLARIS confocal platform. We will detail the experimental design and considerations for executing high-multiplex experiments with 15+ fluorophores on a single sample, including the selection of optimized panels and the inherent challenges associated with these approaches. We will address the technical aspects of imaging high-multiplex samples in a single round of labeling and imaging, demonstrating its application for 3D spatial omics. Our aim is to provide a comprehensive understanding of how these technologies can be leveraged to generate detailed spatial omics data, thereby advancing the field of spatial biology.

 

Learning Objectives