Optimizing Experimental Design for Gene Editing Success: tips and tricks to increase efficiency and improve outcomes

With the promise of targeted gene editing and CRISPR technologies comes increasing experimental complexity and design challenges.  Success in gene editing experiments may depend on many variables, some of which are amenable to optimization that can drive more desirable outcomes. Cleavage position (distance between cut site and edit site), nuclease selection, donor design and preparation, as well as cell culture conditions are the primary determinants of the editing efficiency. Careful consideration of these factors and the inclusion of NHEJ inhibitors and other factors can increase editing efficiency and minimize cellular toxicity issues. This webinar will cover these issues and provide suggestions on experimental design factors based on Dr. Chen’s more than a decade of experience in genome editing using CRISPR-Cas nucleases and ZFNs.

 

 

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