Mitigating the effects of spillover/spread in multicolor panels.

Multicolor flow cytometry is beneficial because it allows us to gain deeper insights from a given biological sample, with fewer repeat markers in each tube and quicker results. In this three-part webinar series, we will identify the fundamental principles of panel design. In the first webinar, we showed how sample biology can inform fluorochromes choice. In this webinar we will focus on the effects of spillover/spread on data resolution and how to overcome them. And in the next webinar, we will focus on the instrument-related factors that can influence panel design. Join us for all three webinars to understand the principles of panel design and how to use them to troubleshoot panels in your lab.

 

Learning Objectives

 

 

 

Flow cytometry enables simultaneous analysis of multiple markers at the single-cell level. With the increase in number of detectable parameters, the design of a multicolor panel can be challenging and requires an understanding of several factors that can influence panel performance. This three-part webinar series will cover the basic pillars of panel design and standardization:

 

Biology—how antigen density and co-expression can affect fluorochrome choice

Fluorochrome—how spillover spread can be predicted and mitigated

Instrument—how instrument standardization can influence data resolution

 

Make sure to join all three talks in the series to learn how you can troubleshoot existing panels in your clinical flow cytometry lab.