Looking Beyond PCR: Isothermal Amplification Webinar

 

 

Limited sample material and insufficient DNA input pose significant challenges for downstream analysis in various laboratory settings. To overcome this issue, isothermal amplification techniques have emerged as effective solutions. This webinar focuses on two commonly used methods: whole genome amplification (WGA) and loop-mediated isothermal amplification (LAMP).

 

WGA, particularly employing the multiple strand displacement amplification (MDA) technique, allows the generation of large quantities of genomic DNA from limited samples or even single cells. The webinar highlights the utilization of EquiPhi29 DNA Polymerase, an engineered phi29 DNA polymerase mutant, to address low DNA input challenges. Through this presentation, attendees will observe the achievement of uniform amplification from single cells while benefiting from time-saving protocols.

 

The webinar also explores LAMP as an affordable and rapid method for detecting minimal amounts of DNA. Notably, LAMP eliminates the requirement for a thermal cycler and is increasingly deployed in robust field settings for prompt diagnosis of plant pathogens or infectious disease agents such as malaria, Zika, or tuberculosis. The session delves into the specific applications of LAMP in molecular diagnostics, shedding light on its efficacy in diverse scenarios.

 

Learning Objectives