Date: December 4, 2020
Time: 9:00am PST, 12:00pm EST
Activation of Myeloid cells and interferon release is an early response to infection. The 3-marker combination (CD169/CD64/HLA-DR) gives an excellent overview of the innate immune response. The flow cytometry assay can effectively differentiate the immune response to bacteria or viruses.
Bacterial stimuli: CD64 overexpression on neutrophils (nCD64).
Viral stimuli: CD169 overexpression on monocytes (mCD169).
Recent stimulus: increased HLA-DR, Overstimulation/Immune exhaustion: decreased HLA-DR.
Further, the assay, using whole blood, is rapid and easy to perform, making this an invaluable tool in clinical research on antibiotic resistance, and studying interferon signaling during infections.
Learning Objectives:
* For Research Use Only. Not for use in diagnostic procedures.
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