Date: February 10, 2021
Time: 7:00am (PST), 10:00 am (EST)
In this talk today we will discuss a new approach to profile influenza proteins. Influenza vaccines typically consist of either attenuated, in-activated virus or virosomes which are virus- like particles, which contain antigenic proteins. The viral potency is normally measured by quantitation of a protein hemagglutinin (HA) which is a glycoprotein embedded in the viral bilayer and on the virus outer surface contained in the virosomes.
The solution demonstrated in this webinar uses commercially available kits and prebuilt cartridges together with capillary electrophoresis (CE) to profile the influenza viral proteins. It is based on the separation of viral proteins by capillary gel electrophoresis with sodium dodecyl sulphate (CE-SDS) which is an application which has traditionally been used in quality control of monoclonal antibodies produced by biopharmaceutical companies for over 15 years.
Learning Objectives
- Understand how CE provides highly resolved separation and detection of individual HA proteins
- Discover how the CE-SDS provides a fast reproducible assay which is 96 Well plate compatible
- Identify how the method can be adapted to the analysis of multiple viral strains
Webinars will be available for unlimited on-demand viewing after live event.