Identification of Frequent Autoreactive B-cell Response in Pancreatic Ductal Adenocarcinoma Using Cell Sorting and Single-Cell Sequencing

B cell responses within pancreatic ductal adenocarcinoma (PDAC) tumors are associated with improved patient outcomes, yet the specific triggering antigens remain largely unknown. In this webinar, Dr. Min Yao will present a methodology that combines cell sorting, single-cell RNA sequencing (scRNA-Seq), and immunoglobulin (Ig) sequencing of tumor-infiltrating immune cells to uncover these antigens. Dr. Yao's study shows evidence of various immune responses, including activated T and B cells, germinal center reactions, and plasma cells producing antibodies targeting intracellular self-antigens in pancreatic cancer tissue. These findings indicate a potential link between chronic inflammation in pancreatic cancer and an enhanced adaptive immune response, which could have implications for treatment development.

 

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