Gene manipulation in primary human immune cells: Successful strategies and applications for CRISPRko, RNAi and beyond

Gene manipulation studies in primary human immune cells can answer important biological questions within a clinically relevant cellular context. In this webinar we explore the use of several gene editing or modulation tools, including CRISPRko, RNAi and base editing, to modify various primary human immune cell types. In addition, we will discuss numerous applications, such as cell therapy, arrayed and pooled screening, for various gene edited primary immune cell types.

Learning Objectives:

1. Understand the usefulness of gene editing and modulation experiments in various primary cell types

2. Understand the different tools available for gene silencing, knockout and editing

3. Understand the potential research and therapeutic applications for gene edited cell lines