Gene inactivation by CRISPRi: Modulate gene expression with PAM-anchored targeting

Knockdown studies are prevalent and powerful ways to illustrate gene function. CRISPRi delivers a new mechanism for gene knockdown, PAM-anchored gene interference without creating double-strand breaks, introducing CRISPR without the cut.

 

CRISPRi harnesses the precision of CRISPR to repress gene function at the transcriptional level. We are proud to introduce the first commercially available complete CRISPRi product line, including synthetic single-guide RNA (sgRNA) and a proprietary repressor construct (dCas9-SALL1-SDS3).

 

CRISPRi-based gene repression relies on a deactivated Cas9 or dCas9 associated with guide RNA. This complex targets a specific DNA sequence and binds to the DNA downstream of a gene's TSS, blocking gene transcription. The functional result is a depletion of the target protein. Our patent-pending repressor fusion, covalently linked to the dCas9 protein, further inhibits target gene expression by recruiting additional proteins involved in chromatin remodeling and gene silencing.

 

CRISPRi is well suited to study any gene and is an ideal platform for simultaneous interrogation of multiple genes. We will share data showing more robust knockdown than demonstrated with existing CRISPRi systems, as well as efficient multiplexing. Importantly, CRISPRi presents another avenue for orthogonal validation studies.

 

Join us for this exciting webinar. We will share information about the development of CRISPRi, experimental strategies for successful implementation, and data on the utility and performance of CRISPRmod.

 

Topics to be covered