A Fast and Reliable Method for SARS-CoV-2 Wastewater Surveillance

Sponsored by: Promega

Speakers

Carlotta Olivero

Professional Health Collaborator
BIOGRAPHY
Subhanjan Mondal

R&D Scientist
BIOGRAPHY

Event Date & Time

Date: November 30, 2021

Time: 7:00am (PDT), 9:00am (CST), 10:00am (EDT), 4:00pm (CET)

Abstract

The current need for SARS-COV-2 wastewater surveillance programs globally requires many laboratories to set up routine wastewater testing workflows. However, existing workflows are often too slow, tedious, or not sensitive enough to detect low viral levels.

We have developed a fast, sensitive, and easy-to-implement wastewater surveillance laboratory workflow that gets from samples to results in less than 4 hours. First, our unique direct nucleic acid capture method efficiently concentrates nucleic acids and removes PCR-inhibitor to achieve consistent and high yield, ideal for downstream PCR and sequencing methods. Then, wastewater-specific RT-qPCR assays are used to detect SARS-CoV-2 levels. These assays are sensitive, inhibitor-tolerant, and include standards and quality controls according to CDC and EU recommendations.

In this webinar, we will highlight the features of these methods, share validation data, and present a real-world example of how the workflow was setup in a public health lab.

Learning Objectives:

Discuss the benefits of a direct nucleic acid capture approach
Explain how a detection kit with built-in quality controls allows for confident data reporting
Describe the benefits of using a workflow specifically designed for wastewater

Webinars will be available for unlimited on-demand viewing after live event.

LabRoots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.

A Fast and Reliable Method for SARS-CoV-2 Wastewater Surveillance

Carlotta Olivero

Subhanjan Mondal