Facilitating transcriptomics of sensitive target cells with gentle cell sorting

DATE: March 18, 2021

TIME:  8:00am PDT

Sequencing of bulk cells, single cells, and nuclei is opening doors in the understanding of complex biological processes. However, the success and reliability of these methods depends strongly on the quality of the sample. A poor sample quality, for example containing a lot of dead cells, debris, and contamination from cell-free DNA and RNA, impairs sequencing results and also the costs of an experiment. In this webinar, Miltenyi Biotec will highlight the importance of gentle, multi-parametric cell sorting prior to genomic analyses, and how to achieve this by using the microchip-based MACSQuant® Tyto® Cell Sorter.

Alexander Jonsson from Uppsala University recently published new insights on the physiology of pancreatic microvascular endothelial cells (MVECs), a highly heterogenous cell type. Endothelial cell attributes have important implications for various biological processes, such as tissue homeostasis, transplantation, and neoplasia. Alexander will explain how he obtained these cells in a gentle way in order to analyze differences between MVECs in the endocrine and exocrine compartments of the pancreas via RNA sequencing. With this study, he was able to show how islet capillaries in the pancreas differ from their exocrine counterparts at the transcriptome level.

Learning objectives:

• Learn which steps in a genomics workflow are most likely to impair sample quality and how to improve them, especially when specific target cells need to be enriched based on multiple parameters
• Find out how critical cell sorting steps can be designed to have less of an impact on cells by using the right technology
• Get to know how gentle cell sorting of primary human pancreatic MVECs allowed for their transcriptomic analysis, and how this supported the hypothesis that adult human pancreatic tissue is continuously repaired and regenerated

Webinars will be available for unlimited on-demand viewing after live event.

LabRoots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.