During this webcast Dr. Mayumi Fujita of the National Institutes for Quantum and Radiological Science and Technology in Japan will address the method of real-time imaging of invading cells using the IncuCyte Live-Cell Analysis System, and describe how this model is useful for many studies.

In previous studies, only 1% of cells in the cultured human pancreatic cancer cell line, PANC-1, were capable of invasion through MatrigelÆ in a transwell invasion assay. This suggested that invasive PANC-1 cells have unique characteristics conferring this phenotype. This was further investigated using a 3D spheroid model of PANC-1, embedded in Matrigel, coupled with IncuCyteÆ Live Cell imaging and analysis to capture the movement of the distinct invading population in real time. The identified, invasive PANC-1 were collected and metabolically characterized by CE-TOFMS, and their metabolic profile compared with whole-culture PANC-1. (Fujita et al. 2017, Cancer Science). The invasive PANC-1 cells were distinct from those of the whole cultured PANC-1, with demonstrated increased consumption of ATP, assumed activation of ATP-generating pathways, and higher arginine utilization by NOS. Although they had higher oxidative stress, the invading cells were also more resistant to it with greater survival upon exposure to H2O2. A reduction of intracellular GSH by BSO inhibited PANC-1 invasiveness. Taken together, these results provide a unique metabolic profile for this invasive PANC-1 cell population, as compared to control cells. Such methodology is readily available for similar assessments of invasive cell phenotypes.

You will learn:

• A method will be provided for the real-time imaging of cancer cell invasion from 3D spheroid using the IncuCyteÆ Live-Cell Analysis System.
• The utility of this model to study cancer cell invasion will be discussed, describing methods of invasion, including that by single cells, collected cells, elongated invasion, or rounded invasion.
• Important methods information for real-time image capture, identification of invasive phenotypes, and integration with metabolomics analysis.
• How to easily apply the use of IncuCyte technology and associated applications to their cell line of interest for related studies.