Advanced digital PCR applications for cell and gene therapy research: Viral vector quantification and CAR-T cell monitoring

This presentation explores how dPCR enables precise and sensitive quantification of CAR-T cells and how dPCR can be utilized in research for CAR-T cell monitoring. Dr. Garcia will further explore how this technology is driving advancements in cell and gene therapy research for cancer and other diseases.

Accurate quantification of AAV vectors could be crucial for quality control and timely decision-making during drug development. dPCR is a precise and reliable method for quantifying nucleic acid targets without relying on standard curves. The Applied Biosystems™ QuantStudio™ Absolute Q™ AutoRun dPCR Suite simplifies not only the workflow, but also data collection and analysis, enabling high-throughput sample processing with minimal intervention. This presentation outlines a recent study that demonstrated the consistency of dPCR data generated by this suite using an AAV assay, achieving less than 3% coefficient of variation (CV) for all nucleic acid targets within the expected concentration accuracy range. The study also highlights the promising results and importance of high throughput dPCR workflow solutions for gene therapy research and development. .

The QuantStudio Absolute Q dPCR System and QuantStudio Absolute Q AutoRun dPCR Suite are for Research Use Only and are not to be used in diagnostic procedures.