Scientific Liaison and Trainer, Horizon Discovery
BIOGRAPHY
JUN 27, 2018 8:00 AM PDT
Cell line engineering with CRISPR-Cas9 - Tips and tricks to maximize success
Speaker
Event Date & Time
DATE: June 27, 2018
TIME: 08:00AM PDT
Abstract
CRISPR-Cas9 is a versatile tool to discover more about your biological pathway or gene of interest. The simplicity of the CRISPR-Cas9 system has led to an explosion in applications, from single cell genomics to personalized medicine.
After more than 10 years of experience in cell line engineering, we have optimized all steps of the workflow. Here we present some useful tips and tricks that can help minimize the bench time needed to reach your gene-engineering goals.
In this webinar, we will discuss key considerations when embarking on your gene engineering project such as designing reagents, targeting your gene of interest, and validating the resulting clones as well as the benefit of using a secondary model.
Learning Objectives:
• Introduction to CRISPR-Cas9 genome engineering
• Important considerations when initiating your gene engineering projects, including:
- Cell line optimization
- Gene target
- Guide choice and positioning
- Donor design
- Screening strategies and validation
• Methods to rapidly determine genotype
• Confidence in your results with thorough validation of your phenotype
More resources from Horizon:
Find out more about our ready-made Cell Lines, the ideal secondary model for your phenotype validation.
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Cell line engineering with CRISPR-Cas9 - Tips and tricks to maximize success
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