Approaches to sample preparation

Even as next-generation sequencing (NGS) has become widely used, and the costs have been reduced, it remains critical to achieve reliable and robust data at the end of the sequencing run. Sequencing labs have very little control over the quantity and quality of the sample received from the pathology lab or clinic, yet sample quality can have a major impact on the downstream processes.

 

While the manual, column-based approach to nucleic acid isolation is still widely used, scientists under pressure from high-throughput demands are turning their attention to superparamagnetic beads. These are automation friendly and well-suited for a range of applications, including sample preparation for next generation sequencing (NGS). Each technique has pros and cons, and selection will depend on sample type, throughput requirements, targets, and other factors.

 

In this webinar you will: