Applications of multiplex digital PCR: Monitoring wastewater for SARS-CoV-2 and measuring Bacterial Gene Expression

Sponsored by: Thermo Fisher Scientific

Speaker

Event Date & Time

Date: April 06, 2022

Time: 9:00am (PDT), 12:00pm (EDT), 6:00pm (CEDT)

Abstract

Digital PCR is a specialized tool for nucleic acid quantification, which has applications in many research areas including those in microbiology and virology research. Leveraging optical multiplexing, multiple targets expected within a single sample can be tested in a single digital PCR reaction, providing absolute quantification without the need for a reference or standard curve.

In this webinar, invited speaker Dr. Joan L. Slonczewski of Kenyon College will describe their ongoing research, which leverages digital PCR.

Highlights include:

The use of the Applied Biosystems QuantStudio Absolute Q Digital PCR System* alongside the Combinati SARS-CoV-2 Wastewater Surveillance kit to monitor and measure the quantity of SARS-CoV-2 viral RNA from the wastewater influent of a village community including college residence halls—equating to approximately 2,500 village residents overall.
The development and use of a custom quadruplex assay to measure bacterial mRNA expression of multidrug resistance pumps in E. coli.

* For Research Use Only. Not for use in diagnostic procedures.

Learning Objectives

Identify the benefits of using digital PCR for precise nucleic acid target measurement
Describe how multiplex digital PCR can be used as a tool for microbiology and virology research
Recognize the benefits of precise environmental surveillance for emerging pathogen research

Webinars will be available for unlimited on-demand viewing after live event.

Applications of multiplex digital PCR: Monitoring wastewater for SARS-CoV-2 and measuring Bacterial Gene Expression

Joan Slonczewski