DNASTAR offers an integrated suite of software for assembling and analyzing sequence data from all major next-generation sequencing platforms. The software supports a variety of key workflows on a desktop computer and on the Cloud. A new Somatic/Cancer Gene Panel workflow supports several types of data sets, including Ion Torrent AmpliSeqTM Comprehensive Cancer Panel, Illumina TruSight Cancer Panel, as well as custom gene panels.
The workflow will be demonstrated, illustrating how both normal and tumor sample data are input to the system in a single step. The pipeline analyzes the two samples separately calling small indels and single-nucleotide polymorphisms (SNPs) in each sample separately. Upon completion of the variant calling, just a few data filtering steps make comparison between the tumor and normal control sample a straight-forward and efficient process.

Learning objectives:

• Have a better understanding of sequence assembly and analysis software for cancer gene panels.
• Learn how to conduct tumor/normal sequence data analysis.