RNAi Technologies: The term RNA interference (RNAi) was coined to describe a cellular mechanism that use the gene's own DNA sequence of gene to turn it off, a process that researchers call silencing. In a wide variety of organisms, including animals, plants, and fungi,RNAi is triggered by double-stranded RNA (dsRNA).
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There is rapidly growing interest in using the CRISPR-Cas9 system for functional screening, both as a primary screening tool and as an orthogonal tool for RNAi hit validation. High throughput...
The CRISPR-Cas9 system is being widely used for genome engineering in many different biological applications. It was originally adapted from the bacterial Type II CRISPR system and uses a Cas...
This is an introduction to the biological mechanism of RNA interference (RNAi) and experimental application of short, interfering RNA (siRNA). The key steps of the endogenous RNAi pathway wil...