Cell Culture: the process by which cells are grown under controlled conditions, generally outside of their natural environment. Cell culture conditions can vary for each cell type, but artificial environments consist of a suitable vessel with substrate or medium that supplies the essential nutrients (amino acids, carbohydrates, vitamins, minerals), growth factors, hormones, and gases (CO2, O2), and regulates the physio-chemical environment.
Combinatorial inhibition of effector and feedback pathways is a promising treatment strategy for KRAS mutant cancers. However, the particular pathways that should be targeted to optimize the...
CRISPR experiments are a powerful tool which are easy to carry out, however it is more difficult to determine the outcome of these experiments, and to ensure that only the desired targets we...
There are many methods of nucleic acid isolation. Each technology offers different approaches of purification of the template. However, it is important to control carry over of “proces...
For more than a century, breakthroughs in biological sciences have relied on the ability to study cells outside of respective organisms. While majority of cell culturing is still performed u...
Extracellular vesicles (EVs) are lipid bilayer-delimited pieces of cells that are released from the plasma membrane as "ectosomes" and from the endosomal system as "exosomes.&...
Extracellular ligands bind to receptors on the cell surface leading to receptor internalization. Once internalized into small vesicles, the vesicles fuse with an organelle known as the sorti...
Intestinal organoids are self-organizing, 3D structures derived from either pluripotent stem cells or from primary tissues with the abiltiy to recapitulate some of the spatial architecture a...
Extracellular vesicles (EVs) are nanosized vesicles containing active proteins, lipids, and different types of genetic material such as non coding RNA species, related to the characteristics...
Date: Time: Over the last couple of years, it has become apparent that affinity-tuned CAR T cells display enhanced expansion and prolonged persistence in patients and that this strategy can...
Cell culture continues to be a critical tool for most life science research and applications. From HeLa to iPSCs and 3D to organoids, culture methods have become more advanced and techniques...
MicroRNAs (miRs) are small non-coding RNAs whose expression is altered in several types of human cancers. Recent evidence supports their inter-cellular transfer through extracellular vesicle...
Chronic kidney disease (CKD) is a major public health threat, affecting 11-15% of the U.S. population alone. Currently, there are no effective therapies to cure CKD. Drug therapies are not p...
Chronic kidney disease (CKD) is a global health threat, affecting over 10% of the world population, including an estimated 37 million Americans. Importantly, glomerular diseases account for...
Extracellular vesicles (EV) from many cell types have demonstrated therapeutic potential against many different diseases. Inhibiting progress in this area is the capability to produce EVs in...
Learning Objectives: 1. Understand methods of isolating and enriching exosomes along with their pros/cons of the different techniques 2. What do we mean when we talk about subtyping, and wha...
The extracellular matrix (ECM) is a three-dimensional structure that provides physical support for tissues/organs and biochemical/biomechanical cues for tissue morphogenesis, differentiation...
GeoMx Digital Spatial Profiling Technology! The GeoMx Digital Spatial Profiler (DSP) is a novel platform developed by NanoString. This leading digital technology offers multiplexed measureme...
I will present our new computer vision algorithm, ST-Net, which can computationally synthesize spatially resolved transcriptomics directly from H&E histology images (He et al. Nature Bio...
Reactive oxygen species (ROS) are well-known for their detrimental effects leading to oxidative stress, cell death, aging, and degenerative disorders. However, there is increasing evidence t...
DATE: September 22, 2020 TIME: 7am PDT, 10am EDT, 4pm CEST Shakers and bioreactors are established versatile systems for cultivating microorganism, plant, and animal cells......
Learning Objectives: 1. Understanding the integral value of collaboration for advancement in scientific research; how to establish and grow academic, Pharma, and industry connections 2. Lear...
Image-based high-content screening (HCS) involving cancer cell lines grown in conventional two-dimensional (2D) cell culture, has provided a cornerstone assay for searching new agents to tre...
While live cell imaging offers advantages over traditional static imaging, this approach has been challenging for studying microbes due to the difficulty in tracking very small cells in a si...
Date: September 10, 2020 Time: 9:00am (PDT), 12:00pm (EDT) Osmolality testing is relevant throughout the entire bioprocessing workflow. As customers look to refine mAb and gene therapy workf...
Combinatorial inhibition of effector and feedback pathways is a promising treatment strategy for KRAS mutant cancers. However, the particular pathways that should be targeted to optimize the...
CRISPR experiments are a powerful tool which are easy to carry out, however it is more difficult to determine the outcome of these experiments, and to ensure that only the desired targets we...
There are many methods of nucleic acid isolation. Each technology offers different approaches of purification of the template. However, it is important to control carry over of “proces...
For more than a century, breakthroughs in biological sciences have relied on the ability to study cells outside of respective organisms. While majority of cell culturing is still performed u...
Extracellular vesicles (EVs) are lipid bilayer-delimited pieces of cells that are released from the plasma membrane as "ectosomes" and from the endosomal system as "exosomes.&...
Extracellular ligands bind to receptors on the cell surface leading to receptor internalization. Once internalized into small vesicles, the vesicles fuse with an organelle known as the sorti...
Intestinal organoids are self-organizing, 3D structures derived from either pluripotent stem cells or from primary tissues with the abiltiy to recapitulate some of the spatial architecture a...
Extracellular vesicles (EVs) are nanosized vesicles containing active proteins, lipids, and different types of genetic material such as non coding RNA species, related to the characteristics...
Date: Time: Over the last couple of years, it has become apparent that affinity-tuned CAR T cells display enhanced expansion and prolonged persistence in patients and that this strategy can...
Cell culture continues to be a critical tool for most life science research and applications. From HeLa to iPSCs and 3D to organoids, culture methods have become more advanced and techniques...
MicroRNAs (miRs) are small non-coding RNAs whose expression is altered in several types of human cancers. Recent evidence supports their inter-cellular transfer through extracellular vesicle...
Chronic kidney disease (CKD) is a major public health threat, affecting 11-15% of the U.S. population alone. Currently, there are no effective therapies to cure CKD. Drug therapies are not p...
Chronic kidney disease (CKD) is a global health threat, affecting over 10% of the world population, including an estimated 37 million Americans. Importantly, glomerular diseases account for...
Extracellular vesicles (EV) from many cell types have demonstrated therapeutic potential against many different diseases. Inhibiting progress in this area is the capability to produce EVs in...
Learning Objectives: 1. Understand methods of isolating and enriching exosomes along with their pros/cons of the different techniques 2. What do we mean when we talk about subtyping, and wha...
The extracellular matrix (ECM) is a three-dimensional structure that provides physical support for tissues/organs and biochemical/biomechanical cues for tissue morphogenesis, differentiation...
GeoMx Digital Spatial Profiling Technology! The GeoMx Digital Spatial Profiler (DSP) is a novel platform developed by NanoString. This leading digital technology offers multiplexed measureme...
I will present our new computer vision algorithm, ST-Net, which can computationally synthesize spatially resolved transcriptomics directly from H&E histology images (He et al. Nature Bio...
Reactive oxygen species (ROS) are well-known for their detrimental effects leading to oxidative stress, cell death, aging, and degenerative disorders. However, there is increasing evidence t...
DATE: September 22, 2020 TIME: 7am PDT, 10am EDT, 4pm CEST Shakers and bioreactors are established versatile systems for cultivating microorganism, plant, and animal cells......
Learning Objectives: 1. Understanding the integral value of collaboration for advancement in scientific research; how to establish and grow academic, Pharma, and industry connections 2. Lear...
Image-based high-content screening (HCS) involving cancer cell lines grown in conventional two-dimensional (2D) cell culture, has provided a cornerstone assay for searching new agents to tre...
While live cell imaging offers advantages over traditional static imaging, this approach has been challenging for studying microbes due to the difficulty in tracking very small cells in a si...
Date: September 10, 2020 Time: 9:00am (PDT), 12:00pm (EDT) Osmolality testing is relevant throughout the entire bioprocessing workflow. As customers look to refine mAb and gene therapy workf...
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