mouse monoclonal IgG2a; PCNA Antibody (PC10) is an IgG2a κ mouse monoclonal PCNA antibody (also designated ATLD2 antibody) that detects the PCNA protein of m, r, h, insect, S. pombe origin by WB, IP, IF, IHC(P) and FCM. PCNA Antibody (PC10) is available as both the non-conjugated anti-PCNA antibody form, as well as multiple conjugated forms of anti-PCNA antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. The PCNA (proliferating cell nuclear antigen) protein is synthesized in early G1 and S phases of the cell cycle, functions in cell cycle progression, DNA replication and DNA repair. In early S phase, the PCNA protein exhibits granular distribution and is absent from the nucleoli; however, in late S phase, it relocates to the nucleoli. Thus, in staining applications, the PCNA Antibody exhibits nuclear staining. PCNA exists in two basic forms: one involved in ongoing DNA replication, which localizes specifically to the nucleus, and a second, soluble form, not implicated in constant synthesis. Interestingly, the latter form degrades in the presence of organic solvents, rendering it undetectable by histological methods in tissues using organic fixatives. Thus, only the synthesizing form is detectable by the PCNA antibody using this method.