mouse monoclonal IgG1; NOS1 Antibody (A-11) is an IgG1 κ mouse monoclonal NOS1 antibody (also designated bNOS antibody or nNos antibody) that detects the NOS1 protein of mouse, rat and human origin by WB, IP, IF and ELISA. NOS1 Antibody (A-11) is available as both the non-conjugated anti-NOS1 antibody form, as well as multiple conjugated forms of anti-NOS1 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Nitric oxide (NO) has a broad range of biological activities and has been implicated in signaling pathways in phylogenetically diverse species. Nitric oxide synthases (NOSs), the enzymes responsible for synthesis of NO, contain an N-terminal oxygenase domain and a C-terminal reductase domain. NOS activity requires homodimerization as well as three cosubstrates (L-arginine, NADPH and O2) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin and heme). Several distinct NOS isoforms have been described and been shown to represent the products of three distinct genes. These include two constitutive Ca2+/CaM-dependent forms of NOS, including NOS1 (also designated ncNOS) whose activity was first identified in neurons, and NOS3 (also designated ecNOS), first identified in endothelial cells. The inducible form of NOS, NOS2 (also designated iNOS), is Ca2+-independent and is expressed in a broad range of cell types.