mouse monoclonal IgG1; Dnmt2 Antibody (D-9) is an IgG1 κ mouse monoclonal Dnmt2 antibody (also designated DNMT2 antibody, or DNA methyltransferase 2 antibody) that detects the Dnmt2 protein of mouse, rat and human origin by WB, IP, IF and ELISA. Dnmt2 Antibody (D-9) is available as both the non-conjugated anti-Dnmt2 antibody form, as well as multiple conjugated forms of anti-Dnmt2 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Methylation at the 5'-position of cytosine is the only known naturally occurring covalent modification of the mammalian genome. DNA methylation requires the enzymatic activity of DNA 5-cytosine methyltransferase (Dnmt) proteins, which catalyze the transfer of a methyl group from S-adenosyl methionine to the 5'-position of cytosines residing in the dinucleotide CpG motif, and this methylation results in transcriptional repression of the target gene. The Dnmt enzymes are encoded by independent genes. Dnmt1 is the most abundant, and it preferentially methylates hemimethylated DNA and coordinates gene expression during development. Additional mammalian Dnmt proteins include Dnmt2 and Dnmt3. Dnmt2 lacks the large N-terminal regulator domain of Dnmt1, is expressed at substantially lower levels in adult tissues, and is likely involved in methylating newly integrated retroviral DNA. Dnmt3a and Dnmt3b are encoded by two distinct genes, but both are abundantly expressed in embryonic stem cells, where they also methylate CpG motifs on DNA.