mouse monoclonal IgG1; V5-Probe Antibody (H-9) is an IgG1 κ mouse monoclonal V5-Probe antibody (also designated V5 tag antibody, Simian Virus 5 tag antibody, or SV5 antibody) that detects the V5-Probe protein of origin by WB, IP, IF and ELISA. V5-Probe Antibody (H-9) is available as both the non-conjugated anti-V5-Probe antibody form, as well as multiple conjugated forms of anti-V5-Probe antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Plasmid vectors for the expression of coding regions of eukaryotic genes in bacterial, insect and mammalian hosts are in common usage; such expression vectors are frequently used to encode hybrid fusion proteins consisting of a eukaryotic target protein and a specialized region designed to aid in the purification and visualization of the target protein. An example is the V5-Probe which recognizes a small epitope, termed Pk, on the P/V proteins of the paramyxovirus simian virus 5 (SV5). This small peptide has proven useful in visualization and immunoaffinity purification of expressed fusion proteins. More than 20 recombinant proteins, some of which include transmembrane and secreted proteins, have been tagged with this epitope and detected via western blot, immunoprecipitation and immunofluorescence.