Reading through huge amounts of published qPCR data to select the best experimental framework for your objectives requires a significant amount of time, expertise and resources. Wouldn’t it be great to have a guide packed full of pro tips on how to build and troubleshoot qPCR assays, right at your fingertips? Level up your qPCR assay with advice from the pros. This guide provides you with specific considerations for assay optimisation and validation based on practical application of the Minimum Information for Publication of Quantitative Real-Time (RT) PCR Experiments (MIQE) guidelines.
With this guide you will:
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Learn how to optimise your assay by evaluating and adjusting design elements to improve performance
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Assess the key performance parameters that impact the assay’s efficiency, technical dynamic range and reproducibility
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Receive recommendations for precise characterisation of assay sensitivity, specificity and precision
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Leverage a combination of normalisation methods to address challenges at every step of the protocol
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Get our experts' tips on data analysis in qPCR