The quest to decipher the complex molecular events underpinning cell polarity and organelle segregation during eukaryotic cell division presents a formidable challenge. A predominant focus on metazoans, fungi, and microbial eukaryotes risks neglecting evolutionary insights and unique features inherent to diverse organisms. This narrow scope could impede the broader understanding of protein functions across the eukaryotic kingdom. Our research aims to bridge this gap by exploring universal mechanisms of cell division in an apicomplexan protist. Building on our previous work, which utilized 3D super-resolution expansion microscopy, we have hypothesized that dynamic tethers to the nucleus are key to the cellularization of multinucleated cells. We will further this investigation by scrutinizing the molecular composition and architecture of the centriolar plaque, particularly its role in establishing cell polarity and driving organelle segregation in atypical eukaryotic cell division. Our approach integrates genetic, biochemical, and advanced expansion microscopy assays to pinpoint critical proteins and map their interactions in these vital processes. This research not only promises to broaden the imaging toolkit for microorganism studies but also seeks to uncover novel cell division mechanisms involving conserved nuclear proteins and Microtubule Organizing Centers (MTOCs). This project has the potential to significantly advance basic scientific knowledge and contribute to global health innovations, offering insights into the universality and diversity of cell division processes across the eukaryotic spectrum.

Learning Objectives: 

1. Discuss atypical cell division of a singled cell eurokaryote.

2. Explain advanced imaging for microorganisms - What is Expansion Microscopy.

3. Analyze the new avenues of basic research to contribute to Malaria eradication.